I'm excited to share this paper/video my colleagues and I at the Dillin lab have been working on. It's a JoVE paper explaining how to perform different stress-response assays in the nematode C. elegans. We use these methods to interrogate the activation and function of the ER UPR, mitochondrial UPR, HSR and oxidative stress response. One of the cool things is that these methods can be used for large genetic and pharmacological screens, making worms a great model organism to work with. We have been using these fluorescent reporters for genetic screening, whether using a microscope or a large particle worm-sorter. Importantly, this system can be a great tool for science education, as it can be utilized with minimum equipment.
It was a great pleasure to work together and write this "Protocol book", which since writing has been super useful in guiding new students who join our lab through the basics (and the details) of methods we use routinely in our lab. I hope this paper is useful for the C. elegans community in general, and for young researchers of stress responses in particular. In the future, I hope to implement some of the methods described here in science education programs, allowing students to experience and observe first-hand, and very quickly, how a perturbation of interest (whether genetic or environmental), can affect cellular pathways.
A link to the paper: https://www.jove.com/video/61001/measurements-of-physiological-stress-responses-in-c-elegans
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